Journal: Central-European Journal of Immunology

Article Title: Comparative analysis of PD-L1 expression and tumor-infiltrating lymphocytes between primary breast cancer and matched metastatic lesions: implications for immunotherapy

doi: 10.5114/ceji.2025.149541

Figure Lengend Snippet: Immunohistochemical comparison of immune markers in matched primary and metastatic breast cancer specimens. Representative images showing CD3, CD4 expression in paired primary breast tumor (left panels) and liver metastasis (right panels) from a single patient with basal-like subtype. Original magnification 400× Immunohistochemical comparison of immune markers in matched primary and metastatic breast cancer specimens. Representative images showing CD8, Foxp3, and PD-L1 expression in paired primary breast tumor (left panels) and liver metastasis (right panels) from a single patient with basal-like subtype. Original magnification 400×

Article Snippet: IHC was performed using primary antibodies against: estrogen receptor (ER) (1 : 150, clone OTI1B1, ZSBIO, China), progesterone receptor (PR) (1 : 150, clone I5E5, ZSBIO), HER2 (HercepTest, ZSBIO), CD3 (1 : 300; clone OTI3E10, ZSBIO), CD4 (1 : 50; clone B486A1, ZSBIO), CD8 (1 : 150, clone OTI3H6; ZSBIO), Foxp3 (1 : 100; clone 236A/E7; Talent Biomedical, China), and PD-L1 (1 : 500; Clone OTI9E12, ZSBIO).

Techniques: Immunohistochemical staining, Comparison, Expressing

Journal: American Journal of Translational Research

Article Title: Changes in the IL-18, IL-22, and T lymphocyte subset levels in patients with hepatitis B-related liver cirrhosis and their predictive values for hepatorenal syndrome

doi:

Figure Lengend Snippet: Comparison of the T lymphocyte subsets in the peripheral blood between the two groups before treatment. The concentrations of (A) CD3+ and (B) CD4+ cells and (D) CD4+/CD8+ ratio in the peripheral blood of group A were significantly higher than those in the blood of group B, whereas (C) the concentration of CD8+ cells in group A was significantly lower than that in group B. (E) Flow cytometry results. *P<0.05, ***P<0.001. CD, cluster of differentiation.

Article Snippet: Subsequently, 50 μL of the biotin-labeled antibodies (CD3-FITC [Cat. No. 11-0037-42], CD4-FITC [Cat. No. 11-0041-82], and CD8-PE-Texas Red [Cat. No. MHCD0817] Monoclonal Antibodies) purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. were added.

Techniques: Comparison, Concentration Assay, Flow Cytometry

Journal: American Journal of Translational Research

Article Title: Changes in the IL-18, IL-22, and T lymphocyte subset levels in patients with hepatitis B-related liver cirrhosis and their predictive values for hepatorenal syndrome

doi:

Figure Lengend Snippet: Correlation of the IL-18, IL-22, and T lymphocyte subset levels with the Child-Pugh score. The concentrations of (A) IL-18, (B) IL-22, and (E) CD8+ increased as the Child-Pugh score increased (positive correlation). The (C) CD3+, (D) CD4+, and (F) CD4+/CD8+ decreased as the Child-Pugh score increased (negative correlation). (G) Flow cytometry results. *P<0.05, **P<0.01 and ***P<0.001. IL, interleukin; CD, cluster of differentiation.

Article Snippet: Subsequently, 50 μL of the biotin-labeled antibodies (CD3-FITC [Cat. No. 11-0037-42], CD4-FITC [Cat. No. 11-0041-82], and CD8-PE-Texas Red [Cat. No. MHCD0817] Monoclonal Antibodies) purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. were added.

Techniques: Flow Cytometry

Journal: American Journal of Translational Research

Article Title: Changes in the IL-18, IL-22, and T lymphocyte subset levels in patients with hepatitis B-related liver cirrhosis and their predictive values for hepatorenal syndrome

doi:

Figure Lengend Snippet: Comparison of the T lymphocyte subset levels in the peripheral blood of group B before and after treatment. Based on the results of flow cytometry, (A) CD3+, (B) CD4+, and (D) CD4+/CD8+ increased significantly, whereas (C) CD8+ cells significantly decreased in the peripheral blood of group B. (E) Flow cytometry results. ***P<0.001. CD, cluster of differentiation.

Article Snippet: Subsequently, 50 μL of the biotin-labeled antibodies (CD3-FITC [Cat. No. 11-0037-42], CD4-FITC [Cat. No. 11-0041-82], and CD8-PE-Texas Red [Cat. No. MHCD0817] Monoclonal Antibodies) purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. were added.

Techniques: Comparison, Flow Cytometry

Journal: American Journal of Translational Research

Article Title: Changes in the IL-18, IL-22, and T lymphocyte subset levels in patients with hepatitis B-related liver cirrhosis and their predictive values for hepatorenal syndrome

doi:

Figure Lengend Snippet: Comparison of IL-18, IL-22, and T lymphocyte subset levels in the peripheral blood between patients with and without HRS

Article Snippet: Subsequently, 50 μL of the biotin-labeled antibodies (CD3-FITC [Cat. No. 11-0037-42], CD4-FITC [Cat. No. 11-0041-82], and CD8-PE-Texas Red [Cat. No. MHCD0817] Monoclonal Antibodies) purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. were added.

Techniques: Comparison

Journal: American Journal of Translational Research

Article Title: Changes in the IL-18, IL-22, and T lymphocyte subset levels in patients with hepatitis B-related liver cirrhosis and their predictive values for hepatorenal syndrome

doi:

Figure Lengend Snippet: Predictive value of the peripheral blood IL-18, IL-22, and T lymphocyte subset values for HRS

Article Snippet: Subsequently, 50 μL of the biotin-labeled antibodies (CD3-FITC [Cat. No. 11-0037-42], CD4-FITC [Cat. No. 11-0041-82], and CD8-PE-Texas Red [Cat. No. MHCD0817] Monoclonal Antibodies) purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. were added.

Techniques:

Journal: American Journal of Translational Research

Article Title: Changes in the IL-18, IL-22, and T lymphocyte subset levels in patients with hepatitis B-related liver cirrhosis and their predictive values for hepatorenal syndrome

doi:

Figure Lengend Snippet: Logistic regression analysis for risk factors of HRS

Article Snippet: Subsequently, 50 μL of the biotin-labeled antibodies (CD3-FITC [Cat. No. 11-0037-42], CD4-FITC [Cat. No. 11-0041-82], and CD8-PE-Texas Red [Cat. No. MHCD0817] Monoclonal Antibodies) purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. were added.

Techniques: Concentration Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Immune dysregulation in endometrial tuberculosis: elevated HLA-G and IL-1Ra as key modulators

doi: 10.3389/fcimb.2025.1548238

Figure Lengend Snippet: Illustrates immune cell infiltration and statistical analysis among the three groups. Immune cells, including CD3+ T cells, CD4+ T cells, CD8+ T cells, CD20+ B cells, and CD68+ macrophages, were identified using immunohistochemical staining. (A) Comparison between the PTB group (P) and the ETB lesion group (F). (B) Comparison between the PTB group (P) and the overall ETB group (E). (C) Comparison between the ETB lesion group (F) and its surrounding tissues (S). Scale bar: 200 μm; magnification: immunohistochemical staining (200×).

Article Snippet: The antibodies used in this study are as follows: CD3 (RabMAb, ZA-O503), CD4 (mouse monoclonal antibody, ZM-0418), CD8 (RabMAb, ZA-0508), CD20 (mouse monoclonal antibody, ZM-o039RUO), and CD68 (mouse monoclonal antibody, ZM-0o60), all purchased from OriGene Technologies, China.

Techniques: Immunohistochemical staining, Staining, Comparison